کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212448 1494080 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of phylloquinone and menaquinones in feces, serum, and food by high-performance liquid chromatography–mass spectrometry
ترجمه فارسی عنوان
اندازه گیری فیلوووینون و مناوینون ها در مدفوع، سرم و غذا با استفاده از اسپکترومتری جرمی کروماتوگرافی مایع
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Vitamin K vitamers are abundant in multiple biological matrices.
• A novel LC–MS method was developed for concurrently measuring 11 vitamin K vitamers.
• The method successfully quantified vitamin K vitamers in food, serum and feces.

Vitamin K, comprising phylloquinone (PK) and menaquinones (MKn), is a family of vitamers found in multiple biological and environmental matrices. Advancing emerging evidence for novel and distinct physiologic roles of these vitamers in human health and disease necessitates sensitive and selective methods for quantifying PK and MKn in these matrices. We developed a novel method employing high-performance liquid chromatography–mass spectrometry with atmospheric pressure chemical ionization (LC–APCI–MS) for simultaneous quantification of 11 vitamin K vitamers that can be applied in feces, serum and food. Minimal detectable concentrations of vitamin K vitamers ranged from 1 pmol/g to 30 pmol/g. Limits of quantification ranged from 5 pmol/g to 90 pmol/g. Inter-assay and intra-assay variations were <17% and <8%, respectively, in food, and <12% and <8%, respectively, in feces. Recovery exceeded 80% for all vitamers in both food and feces. The method successfully quantified PK and MKn concentrations in rat chow, feces and serum. In summary, this LC–APCI–MS method provides a sensitive and selective tool for quantifying vitamin K vitamers in feces, serum and food. This method can be applied in human and animal studies examining the role of vitamin K vitamers derived from the diet and gut bacteria synthesis in health and disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 963, 15 July 2014, Pages 128–133
نویسندگان
, , , , ,