کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1213952 | 1494129 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development and validation of a sensitive U-HPLC–MS/MS method with electrospray ionization for quantitation of ranolazine in human plasma: Application to a clinical pharmacokinetic study Development and validation of a sensitive U-HPLC–MS/MS method with electrospray ionization for quantitation of ranolazine in human plasma: Application to a clinical pharmacokinetic study](/preview/png/1213952.png)
A simple, sensitive and high-throughput ultra high-performance liquid chromatography electrospray ionization mass spectrometry (U-HPLC–ESI-MS/MS) method has been developed and validated for the determination of ranolazine in human plasma. Propafenone was employed as the internal standard (I.S.). The analytes were chromatographically separated on a BEH C18 column (50 mm × 2.1 mm, 1.7 μm) with a mobile phase consisting of acetonitrile and aqueous ammonium acetate solution (0.06% formic acid, 7.5 mmol L−1 ammonium acetate, 40:60, v/v). Detection of the analytes was achieved using positive ion electrospray ionization via multiple reactions monitoring mode. The mass transitions were m/z 428.3 → 279.3 for ranolazine and m/z 342.4 → 115.9 for propafenone. The assay was linear over the concentration range 1–3000 ng mL−1, with correlation coefficients ≥0.997. The intra- and inter-day coefficients of variation were less than 8.9% in terms of relative standard deviation and accuracy ranged from 93.0 to 108.9% at all quality control levels. The validated method was a simple sample preparation procedure and short run-time (<2.0 min) method, which was successfully applied to a phase I pharmacokinetic study of ranolazine in Chinese healthy volunteers.
► The analytes of interest were well separated.
► The method is simple, good specificity, robust and time efficient.
► Successfully applied to pharmacokinetic study in healthy volunteers.
Journal: Journal of Chromatography B - Volume 901, 15 July 2012, Pages 119–124