کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1214295 966927 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography–tandem mass spectrometry to affinity purification followed by enzyme immunoassay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography–tandem mass spectrometry to affinity purification followed by enzyme immunoassay
چکیده انگلیسی

A new analytical method suitable for high throughput measurements of LTE4 in human urine is described. The methodology utilizes on-line enrichment and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The novel LC/MS/MS method is rapid, linear from 5 to 500 pg/ml in spiked urine samples of both healthy and asthmatic subjects and more accurate and precise than enzyme immunoassay (EIA) and previous LC/MS/MS methods. Results from sample integrity experiments and preliminary values of urinary LTE4 from healthy adults and children are reported.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issue 27, 1 October 2009, Pages 3169–3174
نویسندگان
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