کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215111 1494152 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of ascorbic acid in single rat peritoneal mast cells using capillary electrophoresis with electrochemical detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Measurement of ascorbic acid in single rat peritoneal mast cells using capillary electrophoresis with electrochemical detection
چکیده انگلیسی

In this paper, the amount of ascorbic acid (AA) in single rat peritoneal mast cell was determined by the method of capillary electrophoresis (CE) with electrochemical detection (ED) at a carbon fiber microdisk bundle electrode. The CE–ED system and the single-cell injection system were rearranged to make the operation more convenient and efficient. In the experiment, a self-made holder made of foam was used to keep the capillary from swing, which kept the stability of the baseline of the electropherogram. The single cell was lysed completely within 5 s using the 0.1% sodium dodecylsulfate (SDS) as the cell lysis solution together with the lysis voltage of 2 kV. The quantitation analysis was accomplished by the use of calibration curves, and the amount of AA in single rat peritoneal mast cell was from 2.4 to 7.1 fmol.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 878, Issues 15–16, 1 May 2010, Pages 1093–1097
نویسندگان
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