Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference

Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.