کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216043 1494160 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Organic solvent extraction and metabonomic profiling of the metabolites in erythrocytes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Organic solvent extraction and metabonomic profiling of the metabolites in erythrocytes
چکیده انگلیسی

We used erythrocytes as the model tissue to evaluate an optimal solution for the extraction of intracellular metabolites and time-dependent variation of the metabolome in living cells. Projection to latent structure (PLS) of the GC/MS and LC/MS data suggested that the most efficient solution for the extraction of metabolites from wet erythrocytes (50 mg) could be a methanol–chloroform–water mixture (950 μL, 700:200:50, v/v/v). PLS-discriminant analysis (DA) clearly profiled a time-dependent alternation of metabolic phenotype of erythrocytes. Identification of the metabolites showed that the process was characterized by accumulating of metabolic products and depleting of nutritious substances in erythrocytes during incubation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issues 18–19, 15 June 2009, Pages 1751–1757
نویسندگان
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