کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1216409 | 967010 | 2006 | 7 صفحه PDF | دانلود رایگان |
The potential importance of the methylation cycle during embryonic development necessitates the establishment of methodology to detect alterations in the relative abundance of s-adenosylmethionine (SAM) and s-adenosylhomocysteine (SAH) in an embryonic experimental system. We have developed a precise and sensitive method for measurement of SAM and SAH based on liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) in single neurulation-stage mouse embryos. Use of a penta-fluorinated high-performance liquid chromatography (HPLC) stationary phase gave enhanced sensitivity due to optimal ionisation in organic mobile phase and increased retention time compared to standard reversed-phase separation. Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02–25.0 μM, SAH 0.01–10.0 μM) were linear (r2 > 0.997) with limits of detection for SAM and SAH of 10 and 2.5 nmol/L, respectively.
Journal: Journal of Chromatography B - Volume 844, Issue 1, 21 November 2006, Pages 112–118