کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217086 1494177 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel HPLC fluorescence method for the quantification of methylphenidate in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel HPLC fluorescence method for the quantification of methylphenidate in human plasma
چکیده انگلیسی

A number of analytical methods have been established to quantify methylphenidate (MPH). However, to date no HPLC methods are applicable to human pharmacokinetic studies without the use of mass spectrometry (MS) detection. We developed a sensitive and reliable HPLC-fluorescence method for the determination of MPH in human plasma using 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride (DIB-Cl) as the derivatizing agent. An established GC-MS method was adopted in this study as a comparator assay. MPH was derivatized using DIB-Cl, and separated isocratically on a C18 column using a HPLC system with fluorescence detection (λex = 330 nm, λem = 460 nm). The lower limit of quantification was found to be 1 ng/mL. A linear calibration curve was obtained over the concentrations ranging from 1 ng/mL to 80 ng/mL (r = 0.998). The relative standard deviations of intra-day and inter-day variations were ≤9.10% and ≤7.58%, respectively. The accuracy ranged between 92.59% and 103.06%. The method was successfully applied to the pharmacokinetic study of a subject who received a single oral dose (0.3 mg/kg) of immediate-release MPH and yielded consistent results with that of the GC-MS method. This method is the first HPLC assay with non-MS detection providing sufficient reliability and sensitivity for both pre-clinical and clinical studies of MPH.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 858, Issues 1–2, 15 October 2007, Pages 91–95
نویسندگان
, , , , , ,