کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217090 1494177 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive and selective liquid chromatography–tandem mass spectrometry method for the quantification of aniracetam in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive and selective liquid chromatography–tandem mass spectrometry method for the quantification of aniracetam in human plasma
چکیده انگلیسی

A rapid, sensitive and selective LC–MS/MS method was developed and validated for the quantification of aniracetam in human plasma using estazolam as internal standard (IS). Following liquid–liquid extraction, the analytes were separated using a mobile phase of methanol–water (60:40, v/v) on a reverse phase C18 column and analyzed by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode using the respective [M + H]+ ions, m/z 220 → 135 for aniracetam and m/z 295 → 205 for the IS. The assay exhibited a linear dynamic range of 0.2–100 ng/mL for aniracetam in human plasma. The lower limit of quantification (LLOQ) was 0.2 ng/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The validated LC–MS/MS method has been successfully applied to study the pharmacokinetics of aniracetam in healthy male Chinese volunteers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 858, Issues 1–2, 15 October 2007, Pages 129–134
نویسندگان
, , , , ,