کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217147 | 1494185 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous determination of fluoroquinolones and tetracyclines in chicken muscle using HPLC with fluorescence detection Simultaneous determination of fluoroquinolones and tetracyclines in chicken muscle using HPLC with fluorescence detection](/preview/png/1217147.png)
A multiresidue method has been developed which allows for the simultaneous determination of both fluoroquinolones and tetracyclines in chicken muscle. Samples were extracted with a mix of acetonitrile and 0.1 M citrate, 150 mM MgCl2, pH 5.0. After centrifugation and evaporation, the extracts could be analyzed by liquid chromatography with fluorescence detection. Good recoveries (63–95%) were obtained from samples fortified with a mix of five fluoroquinolones and three tetracyclines, with satisfactory relative standard deviations. Limits of detection were 0.5 ng/g (danofloxacin), 1 ng/g (oxytetracycline, ciprofloxacin, enrofloxacin), 1.5 ng/g (tetracycline), 2 ng/g (difloxacin) and 5 ng/g (sarafloxacin, chlortetracycline). Enrofloxacin and its metabolite ciprofloxacin, as well as oxytetracycline were determined in enrofloxacin and oxytetracycline incurred chicken muscle using this method.
Journal: Journal of Chromatography B - Volume 846, Issues 1–2, 1 February 2007, Pages 8–13