کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217373 1494190 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPLC analysis of the second-generation antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
HPLC analysis of the second-generation antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma
چکیده انگلیسی

A liquid chromatographic method with ultraviolet detection was developed for the analysis of the recent antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma. The analytes were separated on a C8 reversed phase column, using a mobile phase composed of acetonitrile and a 12.3 mM, pH 3.0 phosphate buffer containing 0.1% triethylamine (35:65, v/v). Clomipramine was used as the Internal Standard. Using a solid phase extraction procedure with C2 cartridges high extraction yields (>94%) and good purification from matrix interference were obtained. Good linearity was obtained in the 7.5–250.0 ng mL−1 range for sertraline and in the 10–500 ng mL−1 range for N-desmethylsertraline. The analytical method was validated in terms of precision, extraction yield and accuracy. These assays gave R.S.D.% values for precision always lower than 3.9% and mean accuracy higher than 90%. Thanks to its good selectivity, the method proved to be suitable for the analysis of plasma samples from patients treated with sertraline as either monotherapy or polypharmacy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 836, Issues 1–2, 19 May 2006, Pages 116–119
نویسندگان
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