کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1219577 | 967720 | 2015 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Anti-inflammatory effects of 4′-demethylnobiletin, a major metabolite of nobiletin Anti-inflammatory effects of 4′-demethylnobiletin, a major metabolite of nobiletin](/preview/png/1219577.png)
• 4′-Demethylnobiletin (4DN) dose-dependently inhibited lipopolysaccharide (LPS)-induced nitric oxide production in macrophages.
• 4DN inhibited reduced LPS-induced expression of pro-inflammatory proteins, namely, iNOS, COX-2, PGE2, IL-1β and IL-6.
• 4DN inhibited LPS-induced nuclear translocation of NF-κB and AP-1.
• 4DN activated transcription factor Nrf2 and its dependent genes, including HO-1 and NQO1.
Nobiletin, a citrus flavonoid, has been associated with various beneficial biological activities. 4′-Demethylnobiletin (4DN) is a major metabolite of nobiletin, and its tissue level was found to be much higher than that of nobiletin after oral administration of nobiletin in mice. Anti-inflammatory effects of 4DN were studied in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. The results showed 4DN not only dose-dependently inhibited LPS-induced nitric oxide production, but also significantly reduced expression of pro-inflammatory mediators, namely PGE2, IL-1β and IL-6. 4DN potently suppressed the expression of iNOS and COX-2 at both protein and mRNA levels. 4DN also inhibited nuclear translocation of NF-κB and AP-1. Furthermore, we demonstrated that 4DN activated transcription factor Nrf2 and its dependent genes including HO-1 and NQO1 whose expression may contribute to anti-inflammatory effects. The results demonstrated anti-inflammatory effects of 4DN and provided a scientific basis for using nobiletin as a nutraceutical to inhibit inflammation-driven diseases.
Journal: Journal of Functional Foods - Volume 19, Part A, December 2015, Pages 278–287