کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1219584 | 967720 | 2015 | 13 صفحه PDF | دانلود رایگان |
• Onion as a functional ingredient in relation to Wistar rats metabolome was investigated.
• The metabolomic approach integrated results from LC-MS, CE-MS and GC-MS methodologies.
• S-methyl-L-cysteine and S-methylcysteine sulphoxide were identified in the HCO-fed rats.
• Onion supplementation modulated the effects of diet-induced hypercholesterolaemia.
• Mechanism involved methylation cycle, arginine and tryptophan pathways, among others.
The preventive and modulating effects of onion in metabolites affected by a high-cholesterol diet and related to molecular pathway dysfunction were examined. Plasma obtained from 24 male Wistar rats after seven weeks of experimental feeding was analysed through a non-targeted multiplatform metabolomics approach based on LC-MS, CE-MS and GC-MS methods. A cross-comparison of the three metabolic profiles [I) control (C) group, II) high-cholesterol (HC) group and III) high-cholesterol enriched with onion (HCO) group] pointed out two sulphur metabolites, S-methyl-L-cysteine and S-methylcysteine sulphoxide, as markers of onion intake and evidenced the possibilities to regulate key metabolites. Thus, the methylation cycle, arginine and tryptophan pathways, the modulation of 3-methylhistidine, as well as the routes involved in the glycerophospholipids transformation have been highlighted. This study suggests that onion's biologically active compounds take a prominent role in ameliorating hypercholesterolaemia and related complications through the regulation of altered metabolic pathways.
Journal: Journal of Functional Foods - Volume 19, Part A, December 2015, Pages 363–375