کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1220171 | 1494545 | 2016 | 10 صفحه PDF | دانلود رایگان |
• Eight phenolic compounds were identified and isolated in S. tuberosa fruits by HPLC-MS and 2D NMR for the first time.
• Two new natural products were identified in this species.
• The extract of S. tuberosa pulp showed antioxidant, cancer chemopreventive activity and acetylcholinesterase inhibition.
• HPLC-based activity profiling led to the localization of the active compounds directly in the extracts.
• Gallic acid and its derivative were attributed the high antioxidant activity and acetylcholinesterase inhibition.
The methanolic extract of umbu (Spondias tuberosa) presented high antioxidant activities in the DPPH, ABTS and ORAC assays, as well as acetylcholinesterase (AChE) inhibition activity. The dichloromethane extract exhibited cancer chemopreventive activity, with a quinone reductase induction in Hepa1c1c7 cells. The localization of the active compounds was performed by HPLC activity-based profiling, and preliminary structural information was obtained by HPLC-PAD-ESI-MS and UHPLC-TOF-HRMS. The main constituents from the methanolic extract were efficiently isolated in a single step by preparative MPLC-UV. Two new natural products were identified, together with five known compounds. The structures of the compounds were elucidated by 2D NMR and ESI-HRMS. The dichloromethane extract was fractionated by SPE and by semi-preparative HPLC-UV-ELSD. Using this approach, one anacardic acid derivative was isolated. However, this compound was not responsible for QR induction. This study highlights the potential of umbu as an active ingredient for functional food formulations.
Journal: Journal of Functional Foods - Volume 21, March 2016, Pages 396–405