کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1220228 1494611 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Procedure optimization for extracting short-chain fatty acids from human faeces
ترجمه فارسی عنوان
بهینه سازی روش برای استخراج اسیدهای چرب کوتاه مدت از مدفوع انسانی
کلمات کلیدی
CE، الکتروفورز مویرگی؛ MCE، استرهای سلولز مخلوط؛ SCFA، اسید چرب کوتاه مدت؛ امارات متحده عربی، استخراج اسیدهای چرب زنجیره کوتاه از طریق اولتراسوند ؛ استخراج؛ بهينه سازي؛ مدفوع
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Short-chain fatty acids content in stool were investigated.
• Optimal parameters for the extraction of fatty acids from stool were evaluated.
• Time of ultrasound digestion: 40 min. (at 35 °C) was selected.
• Time of shaking: 4 min was chosen.
• Three subsequent extractions were proposed.

Short-chain fatty acids play an important role in the physiology and metabolism of the colon. Disturbed balance of such compounds in human gut can significantly contribute to etiological factors of various gastrointestinal disorders and it may also increase the risk of developing cancer or cardiovascular diseases. The aim of the study was to select the optimal parameters for acetic, propionic and butyric acids extraction from stool samples. The experimental conditions were optimized with respect to the solvent sample shacking time, sample ultrasounds (Ultrasound-Assisted Extraction, UAE) exposure time and the number of extractions from the particulate stool samples. The screening of experimental parameters was conducted with fractional factorial design of experiments, namely 33–1. The optimal conditions for UAE were found, namely ultrasound digestion time of 40 min (at 35 °C), shaking time of 4 min, and the three subsequent extractions.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 124, 30 May 2016, Pages 337–340
نویسندگان
, , , , , , ,