Analytical methods for kinetic studies of biological interactions: A review

• Various methods are available for kinetic studies of biological interactions.
• Stopped-flow analysis is useful in examining solution-phase interactions.
• Surface plasmon resonance is a flexible tool for kinetic and binding studies.
• Affinity chromatography is a separation-based method for kinetic studies.
• Capillary electrophoresis has been adapted for solution-phase kinetic studies.

The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.

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