کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1220759 1494624 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid authentication of Cordyceps by lateral flow dipstick
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid authentication of Cordyceps by lateral flow dipstick
چکیده انگلیسی


• Dipstick was employed to distinguish O. sinensis from its common adulterant.
• Internal control was included to eliminate false negative conclusion.
• Specific primers designed could differentiate O. sinenisis from two Cordyceps species.

Cordyceps (Dongchongxiacao), a valuable traditional Chinese medicine, is composed of the fruiting body of Ophiocordyceps sinensis (Family: Ophiocordycipitaceae) on a caterpillar of ghost-moth species (Family: Hepialidae). Owing to its multiple potential functions, Cordyceps are in great demand and represent significant economic value. Adulterants or substitutes named Cordyceps or Chongcao from related fungi have been reported. In this study, polymerase chain reaction (PCR) coupled with a lateral flow dipstick (LFD) system was developed to distinguish genuine herb O. sinensis from its common adulterant Cordyceps gunnii and Cordyceps militaris. Specific primers (EF-CS-F1-Biotin, EF-CG-F1-Biotin and EF-CM-F1- Biotin) were designed to differentiate the three Cordyceps species. Internal control (EF-F1-b-DIG and EF-R1-FITC) was included to minimize the false signal due to PCR inhibitors or DNA degradation. LFD was then successfully employed for speedy and accurate detection of the respective PCR products.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 111, 10 July 2015, Pages 306–310
نویسندگان
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