کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1220887 1494627 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening and profiling stilbene-type natural products with angiotensin-converting enzyme inhibitory activity from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Screening and profiling stilbene-type natural products with angiotensin-converting enzyme inhibitory activity from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder
چکیده انگلیسی


• Analysis of 10 stilbene-type compounds in Ampelopsis brevipedunculata var. hancei.
• ACE inhibitors (+)-hopeaphenol and (+)-vitisin A are abundant in the bark.
• Correlation of structure, activity and abundance.

In this study, we screened 10 resveratrol derivatives isolated from Ampelopsis brevipedunculata var. hancei (Planch.) Rehder (ABH) for angiotensin I converting enzyme (ACE) inhibitory (ACEI) activity. Among these compounds, (+)-hopeaphenol and (+)-vitisin A showed the lowest IC50 values (∼1.5 μM) toward ACE. In addition, the compounds’ abundances and distributions in ABH were profiled using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Interestingly, trimers and tetramers of resveratrol were mainly obtained from the bark of ABH when 90% ethanol was used for extraction. This result implies that the antihypertension effect of ABH extract may be mainly contributed by (+)-hopeaphenol (F1) and (+)-vitisin A (F2) in the ABH bark due to their remarkable ACE inhibitions. Moreover, the sizes and structures of these compounds were further correlated to their affinities toward ACE using molecular docking calculations. The results showed that resveratrol tetramers interact with ACE more favorably than other smaller oligomers.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 108, 10 April 2015, Pages 70–77
نویسندگان
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