Two-dimensional liquid chromatography (LC) of phenolic compounds from the shoots of Rubus idaeus ‘Glen Ample’ cultivar variety

• Valuation of 1D and 2D-LC for identification of phenols in Rubus idaeus shoots.
• The setup of C18 and PFP columns for 2D-LC proved viable for separation of phenols.
• Simultaneous separation of ellagitannins, flavonols, flavan-3-ols and phenolic acids.

In this study the application of two-dimensional LC (2D LC) for qualitative analysis of polyphenols and simple phenols in the shoots of Rubus idaeus ‘Glen Ample’ variety is presented. In the preliminary analysis, the methanol extract of the shoots was analyzed by one-dimensional LC. One-dimensional LC separation profiles of phenolics from R. idaeus ‘Glen Ample’ shoots were dependent on column type, mobile phase composition and gradient program used. Two-dimensional LC system was built from connecting an octadecyl C-18 silica column in the first dimension and pentafluorophenyl column in the second dimension, coupled with DAD and MS (ESI, APCI, DUIS ionization) detectors. A total of 34 phenolic compounds belonging to the groups of phenolic acids, ellagitannins, flavan-3-ols, flavonols and ellagic acid conjugates were identified in the shoots of R. idaeus ‘Glen Ample’. The established 2D LC method offers an effective tool for analysis of phenolics present in Rubus species.

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