کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1221025 | 1494662 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Validated method for the determination of misoprostol acid in whole blood by ultra performance liquid chromatography–tandem mass spectrometry Validated method for the determination of misoprostol acid in whole blood by ultra performance liquid chromatography–tandem mass spectrometry](/preview/png/1221025.png)
Misoprostol is a pharmaceutical synthetic compound, analog of prostaglandin E1, frequently used as an abortifacient in not medically supervised or self-induced abortions, particularly in countries with restrictive abortion laws representing a serious public health problem. The aim of this study was to develop and validate a sensitive analytical method for the determination of misoprostol acid in whole blood samples. The samples were prepared by SPE and the chromatographic separation was performed by UPLC–MS/MS using ESI- and MRM mode with an Acquity UPLC® BEH C18 (50 mm × 2.1 mm i.d., 1.7 μm) column using a methanol–ammonium 0.1% solution gradient in a total run time of 7.0 min. The method showed to be selective and linear in range 25–2000 ng/L. The LOD and LOQ were 10 ng/L and 25 ng/L, respectively. The recovery ranged from 89 to 97%. No carryover and significant matrix effect were observed. The intra- and inter-assay precisions and the inter-assay accuracy results were 4.0% and 5.4%, 5.5% and 4.1%, and −1.4% and −2.8%, for the concentrations 50 and 500 ng/L, respectively. The method developed allows the analysis of misoprostol acid in whole blood samples with adequate sensitivity to the concentration range obtained from therapeutic doses. The method was successfully used in a controlled misoprostol administration study and has been applied in our laboratory in the forensic toxicology field.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 71, December 2012, Pages 198–201