کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1221673 1494639 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of oral antidiabetic drugs in human plasma using microextraction by packed sorbent and high-performance liquid chromatography
ترجمه فارسی عنوان
تعیین همزمان داروهای ضد دیابتی خوراکی در پلاسمایی انسان با استفاده از میکرو اکستراکت کردن توسط جاذب بسته و کروماتوگرافی مایع با کارایی بالا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• MEPS was used for sample preparation of antidiabetic drugs in human plasma.
• Experimental design was employed to optimize extraction and desorption steps.
• Fused core column was used and only 2.2 min were needed for chromatographic run.
• The developed method was validated and applied for real samples.

In this study, a simple method using microextraction by packed sorbent and high-performance liquid chromatography with ultraviolet detection for simultaneous determination of chlorpropamide, gliclazide and glimepiride in human plasma was developed and validated. A fractional factorial design and a complete factorial design were applied to evaluate the parameters which could affect the extraction and desorption steps, respectively. All parameters in the extraction step (pH, sample volume, sample dilution and number of aspiration/ejection cycles) and in the desorption step (percentage of acetonitrile in the elution solvent and number of aspirations of elution solvent through the device) were statistically significant (p > 0.05) when recovery was used as response. The developed method allowed the use of small volumes of sample and solvents and rapid separation by using a fused core column (only 2.2 min were needed). This method was fully validated showing selectivity, precision, accuracy and linearity over the range 1.0–50.0 μg mL−1 for chlorpropamide, 1.0–10.0 μg mL−1 for gliclazide and 0.1–1.0 μg mL−1 for glimepiride. Finally, the validated method was applied in the analysis of samples from volunteers containing the three tested analytes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 96, 5 August 2014, Pages 241–248
نویسندگان
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