کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222211 1494666 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of the components of bitespiramycin by liquid chromatography–mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Identification of the components of bitespiramycin by liquid chromatography–mass spectrometry
چکیده انگلیسی

Reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was used to characterize the components of bitespiramycin, a group of 4″-acylated spiramycins produced by bioengineered strains. In total 38 components were characterized in commercial samples, including 12 impurities that had never been reported before and 12 other that were partially characterized. The structures of these unknown compounds were deduced by comparison of their fragmentation patterns with those of known major components. Their ultraviolet spectra were used to confirm the presence of an α-, β-, γ-, δ-unsaturated butadiene in the macrocyclic lactone. Compared with the classical method, LC/ESI-MS/MS is particularly advantageous in terms of sensitivity and efficiency to characterize minor components at trace levels in multi-component antibiotics.


► The impurity profiling of bitespiramycin was characterized by LC/ESI-MS/MS.
► Twelve impurities that had never been reported before were identified.
► Twelve others were partially characterized.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 66, July 2012, Pages 402–410
نویسندگان
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