کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1222362 | 967861 | 2009 | 4 صفحه PDF | دانلود رایگان |
An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe3+/Fe2+ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10−2 mg L−1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L−1.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 49, Issue 2, 20 February 2009, Pages 498–501