Analysis of cycloserine and related compounds using aqueous normal phase chromatography/mass spectrometry

A new approach is evaluated for the analysis of cycloserine, a strongly hydrophilic drug. The method utilized is aqueous normal phase chromatography with a silica hydride-based stationary phase and mass spectrometry for detection. The samples are analyzed to determine the number of components and they are identified when possible. In addition, the composition change is monitored with respect to time and sample solvent. Analyses using both gradient and isocratic conditions are presented. The repeatability of inter- and intraday analyses is also determined.

► Cycloserine is highly polar.
► Aqueous normal phase method is described.
► Analysis done without derivatization or ion-pairing.
► Method can be easily adapted to clinical samples.