کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1222702 | 967870 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and structure characterization of related impurities in etimicin sulfate by LC/ESI-MSn and NMR
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Etimicin sulfate is a semi-synthetic aminoglycoside which is modified from gentamicin C1a, used as an antibiotic effective both to Gram-positive and Gram-negative bacteria infections. Ten impurities were detected in the bulk drug by LC-ELSD and LC-ESI-MSn. Weakly acidic cation exchange resin, CM-sephadex and silica gel column chromatography were used for the isolation of six impurities. Based on LC/ESI-MSn and NMR analysis, related impurities were characterized as gentamicin C1a (1), 1-N-ethyl garamine (2), 2â³-N-ethyl gentamicin C1a, or 6â³-N-ethyl gentamicin C1a (3), 1-N-ethyl-3â²-N-demethyl gentamicin C2b (4), 3-N-ethyl gentamicin C1a (5), 6â³-deamino-6â³-hydroxyl etimicin (6), 1,3-N, N-diethyl garamine (7), 1-N-ethyl gentamicin C2b (8), 2â³ or 6â³-N-ethyl etimicin (9), 1,3-N, N-diethyl gentamicin C1a (10). 10 is a new aminoglycoside, the 1H and 13C NMR data of compounds 5-7, 10 are first reported in the paper. The possible mechanism for the formation of impurities is also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 55, Issue 5, 15 July 2011, Pages 902-907
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 55, Issue 5, 15 July 2011, Pages 902-907
نویسندگان
Hao Wang, Zhijie Zhang, Fei Xiong, Lingyun Wu, Ping Li, Wencai Ye,