کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1222766 | 967871 | 2008 | 8 صفحه PDF | دانلود رایگان |
It was found that the chemiluminescence (CL) produced from the reaction of luminol with iodine in the alkaline medium was strongly inhibited by plant phenolic compounds. Based on this finding, a new flow injection CL method was developed for the determination of caffeic acid and 6′-caffeoylerigeroside. The latter compound was isolated for the first time from Erigeron acris L. herb. The method was simple, rapid and sensitive with a detection limit of 4 × 10−3 ng mL−1 (caffeic acid) and 0.18 ng mL−1 (6′-caffeoylerigeroside), linear range of 0.1–1.5 ng mL−1 (caffeic acid) and 1–200 ng mL−1 (6′-caffeoylerigeroside), relative standard deviation of 3.3% for 10 measurements of 0.45 ng mL−1 caffeic acid and 2.9% for 40 ng mL−1 6′-caffeoylerigeroside. This method was successfully applied to determine the content of phenolic compounds/antioxidant activity of E. acris L. extracts and phenolic acids content in pharmaceutical formulation. A possible mechanism of the inhibition of the proposed CL system was discussed.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 48, Issue 3, 4 November 2008, Pages 579–586