SPE-HPTLC of procyanidins from the barks of different species and clones of Salix

A SPE-HPTLC method was developed for the qualitative and quantitative analysis of procyanidin B1 in willow barks. The chromatography was performed on HPTLC silica gel layer with the mobile phase chloroform–ethanol–formic acid (50:40:6 v/v/v), in the Automatic Developing Chamber—ADC 2. The methanol extracts from willow barks were purified by SPE method on RP-18 silica gel columns with methanol–water (7:93 v/v) as the eluent. The presence of procyanidin B1 was revealed in the majority of investigated willow barks. The content of procyanidin B1 varied from 0.26 mg/g in the extract of Salix purpurea clone 1067–2.24 mg/g in the extract of Salix alba clone 1100. The method was validated for linearity, precision, LOD, LOQ and repeatability.