کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223518 967893 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pharmacokinetic study of a new angiotensin-AT1 antagonist by HPLC
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Pharmacokinetic study of a new angiotensin-AT1 antagonist by HPLC
چکیده انگلیسی

Recently an innovative novel class angiotensin-AT1 antagonist has been developed by Rottapharm. In this study, we present a validated method for detecting CR 3834 in biological matrices using high-performance liquid chromatography (HPLC) with diode array detection. After oral administration (30 mg/kg) to Wistar rats, the plasma and urine concentrations of CR 3834 and its potential metabolic products were determined. Moreover, the plasmatic time course in rats has been determined after intravenous (IV) administration of CR 3834 (5 mg/kg). Biological samples (0.5 ml of plasma and 1 ml of urine) were purified using solid-phase extraction (SPE) of analytes and the internal standard Idebenone, 2,3-dimethoxy-5-methyl-6-(10-hydroxydecyl)-1-4-benzoquinone. A chromatographic separation was performed on an Adsorboshere C18 at 25 °C, with a pre-column of the same matrix; the eluent was made up of acetonitrile/acidified water with CF3COOH (pH 2.01) in ratio of 75:25 (v/v); the flow rate was 1.0 ml/min and a 100 μl loop. The lower limit of detection (LOD) was taken as 25 ng/ml in plasma and 50 ng/ml in urine samples. The lower limit of quantification (LOQ) was taken as 0.1 and 0.2 μg/ml in plasma and urine samples, respectively. The procedures were validated according to international standards with a good reproducibility and linear response (r = 0.9916 in plasma; r = 0.9997 in urine). The coefficients of variation inter assay ranged between 2.579 and 4.951% in plasma, and between 0.813 and 2.460% in urine. Mean recovery for CR 3834 was 79% in plasma and 97% in urine samples. The experiments performed demonstrated that the method presented was suitable for determining this new angiotensin-AT1 antagonist in rat plasma and urine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 48, Issue 2, 29 September 2008, Pages 422–427
نویسندگان
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