کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224703 | 967930 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improved LC of minocycline drug substance Improved LC of minocycline drug substance](/preview/png/1224703.png)
An isocratic liquid chromatographic method is described for the separation of minocycline and its impurities. This method uses XTerra RP-18, 5 μm (25 cm × 4.6 mm I.D.), a silica-based stationary phase with reduced silanol activity. A mobile phase composed of acetonitrile–0.2 M tetrabutylammonium hydrogen sulphate pH 6.5–0.2 M ethylenediaminetetraacetic acid pH 6.5–water (20:20:20:40; v/v/v/v) was used at a flow rate of 1 ml/min. The column temperature was set at 35 °C. UV detection was performed at 280 nm. Optimisation of the separation method and a robustness study were performed by means of a central composite experimental design. The method allows to separate minocycline from known impurities. Some unidentified impurities are also separated. The total time of analysis is less than 20 min.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 4, 3 March 2006, Pages 815–821