کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224705 967930 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High performance liquid chromatographic determination of platinum in blood and urine samples of cancer patients after administration of cisplatin drug using solvent extraction and N,N′-bis(salicylidene)-1,2-propanediamine as complexation reagent
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High performance liquid chromatographic determination of platinum in blood and urine samples of cancer patients after administration of cisplatin drug using solvent extraction and N,N′-bis(salicylidene)-1,2-propanediamine as complexation reagent
چکیده انگلیسی

A high performance liquid chromatographic (HPLC) procedure has been developed for the determination of cisplatin, based on the pre-column derivation of platinum(II) with reagent N,N′-bis(salicylidene)-1,2-propanediamine (H2SA2pn). The neutral platinum complex was extracted, concentrated in an organic solvent and then injected (5 μl) on a reverse phase HPLC column, Varian Micro-Pak SP C-18, 5 μm (150 mm × 4.0 mm i.d.). The complex was eluted isocratically using a ternary mixture of methanol/acetonitrile/water (40/30/30, v/v/v) at a flow rate of 1.0 ml/min and was determined by a UV detector set at 254 nm after elution. A detection limit was found to be 4.0 ng per injection. The amounts of platinum in blood serum and urine of cancer patients after administration of cisplatin were observed in a range of 221–298 ng/ml and 43–97 ng/ml with relative standard deviation (R.S.D.) of 3.6–4.6% and 3.5–4.8%, respectively. Preliminary metabolism profiles of Pt concentrations in blood and urine from the patients were established.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 4, 3 March 2006, Pages 833–839
نویسندگان
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