کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224845 967933 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary electrochromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid separation and determination of structurally related anthraquinones in Rhubarb by pressurized capillary electrochromatography
چکیده انگلیسی

A pressurized capillary electrochromatography (pCEC) with monolithic column has been developed for the rapid separation and determination of five structurally related anthraquinones in Rhubarb. The possibility of rapid separation resulted from the unique pore structure with high permeability and favorable mass transfer characteristics of the monolithic stationary phase. The effect factors such as organic modifier, acidity and concentration of running buffer, separation voltage were investigated to acquire the optimum condition. In the 220 nm wavelengths, the five anthraquinones could be baseline-separated rapidly within 5 min with the separation voltage of −20 kV in 10 mmol/L phosphate buffer (pH 6.2) containing 65% acetonitrile. The calibration graphs of rhein, aloe-emodin, emodin chrysophanol and physcion were linear by plotting the peak area against the analytes concentration over the range of 0.2–65, 0.1–30, 0.1–55, 0.5–30 and 0.5–55 μg/mL, respectively. The detection limits of five anthraquinones were ranged from 0.06 to 0.2 μg/mL and the recoveries of Rhubarb samples were about 81.3–86.4% (R.S.D. ≤ 5.2%). This proposed method was successfully applied to determination of the five analytes in Rhubarb with satisfactory results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 43, Issue 1, 4 January 2007, Pages 352–357
نویسندگان
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