کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225411 968215 2011 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Site-specific proteomic analysis of lipoxidation adducts in cardiac mitochondria reveals chemical diversity of 2-alkenal adduction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Site-specific proteomic analysis of lipoxidation adducts in cardiac mitochondria reveals chemical diversity of 2-alkenal adduction
چکیده انگلیسی

The modification of proteins by lipid peroxidation products has been linked to numerous diseases and age-related disorders. Here we report on the identification of endogenous protein targets of electrophilic 2-alkenals in cardiac mitochondria. An aldehyde/keto-specific chemical labeling and affinity strategy in combination with LC–MS/MS resulted in 39 unique lipoxidation sites on 27 proteins. Several of the target sites were modified by a variety of 2-alkenal products including acrolein, β-hydroxyacrolein, crotonaldehyde, 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal and 4-oxo-2-nonenal. Many of the adduction sites are implicated in the catalytic function of key mitochondrial enzymes suggesting potential impact on pathways and overall mitochondrial function.

Figure optionsDownload high-quality image (78 K)Download as PowerPoint slideHighlights
► Endogenous protein targets of electrophilic 2-alkenals in cardiac mitochondria.
► Aldehyde/keto-specific labeling and affinity strategy in conjunction with LC-MS/MS.
► Distinct target sites were modified by a variety of 2-alkenal products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 11, 19 October 2011, Pages 2417–2429
نویسندگان
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