کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1225957 | 968264 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit](/preview/png/1225957.png)
Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC–ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process.
Figure optionsDownload high-quality image (130 K)Download as PowerPoint slideHighlights
► We analyzed the papaya pulp proteins by 2-DE and MS.
► New information about the protein expression in response to 1-MCP is reported.
► Increasing information of papaya pulp proteins during ripening will be useful for minimizing postharvest losses.
► Proteomics is a useful tool to investigate the ripening processes.
Journal: Journal of Proteomics - Volume 75, Issue 7, 3 April 2012, Pages 2160–2169