کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226003 1494779 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative proteomic analysis of Saccharomyces cerevisiae under different nitrogen sources
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Comparative proteomic analysis of Saccharomyces cerevisiae under different nitrogen sources
چکیده انگلیسی


• Proteome of Saccharomyces cerevisiae with different nitrogen sources were analyzed.
• 169 differentially expressed proteins were detected by 2-DE, 121 of them were identified by MALDI-TOF/TOF.
• The result provides clues that how yeast adapt to different nutritional conditions.
• Proteomics data were compared with previous reported corresponding mRNA data

In cultures containing multiple sources of nitrogen, Saccharomyces cerevisiae exhibits a sequential use of nitrogen sources through a mechanism known as nitrogen catabolite repression (NCR). To identify proteins differentially expressed due to NCR, proteomic analysis of S. cerevisiae S288C under different nitrogen source conditions was performed using two-dimensional gel electrophoresis (2-DE), revealing 169 candidate protein spots. Among these 169 protein spots, 121 were identified by matrix assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF). The identified proteins were closely associated with four main biological processes through Gene Ontology (GO) categorical analysis. The identification of the potential proteins and cellular processes related to NCR offer a global overview of changes elicited by different nitrogen sources, providing clues into how yeast adapt to different nutritional conditions. Moreover, by comparing our proteomic data with corresponding mRNA data, proteins regulated at the transcriptional and post-transcriptional level could be distinguished.Biological significanceIn S. cerevisiae, different nitrogen sources provide different growth characteristics and generate different metabolites. The nitrogen catabolite repression (NCR) process plays an important role for S. cerevisiae in the ordinal utilization of different nitrogen sources. NCR process can result in significant shift of global metabolic networks. Previous works on NCR primarily focused on transcriptomic level. The results obtained in this study provided a global atlas of the proteome changes triggered by different nitrogen sources and would facilitate the understanding of mechanisms for how yeast could adapt to different nutritional conditions.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 101, 14 April 2014, Pages 102–112
نویسندگان
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