Direct analysis of six antibiotics in wastewater samples using rapid high-performance liquid chromatography coupled with diode array detector: A chemometric study towards green analytical chemistry

• Six antibiotics have been determined in effluent and influent wastewater samples.
• MCR/ALS and U-PLS/RBLwere used for modeling of HPLC–DAD data with direct injection.
• Suit dividing of the temporal-wavelength matrix for both algorithms was necessary.
• U-PLS/RBL showed a comparable performance with MCR/ALS for rapid analysispurposes.

In this work, a rapid HPLC–DAD method has been developed for the analysis of six antibiotics (amoxicillin, metronidazole, sulfamethoxazole, ofloxacine, sulfadiazine and sulfamerazine) in the sewage treatment plant influent and effluent samples. Decreasing the chromatographic run time to less than 4 min as well as lowering the cost per analysis, were achieved through direct injection of the samples into the HPLC system followed by chemometric analysis. The problem of the complete separation of the analytes from each other and/or from the matrix ingredients was resolved as a posteriori. The performance of MCR/ALS and U-PLS/RBL, as second-order algorithms, was studied and comparable results were obtained from implication of these modeling methods. It was demonstrated that the proposed methods could be used promisingly as green analytical strategies for detection and quantification of the targeted pollutants in wastewater samples while avoiding the more complicated high cost instrumentations.

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