کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1242031 1495803 2015 5 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free triple-helix aptamer as sensing platform for “signal-on” fluorescent detection of thrombin
ترجمه فارسی عنوان
اپلامر سه گانه ایلام بدون برچسب بدون نیاز به برچسب به عنوان یک پلت فرم سنجش برای سیگنال؟ تشخیص فلورسنت ترومبین
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کلمات کلیدی
سه گانه اپلمر، برچسب رایگان، تشخیص فلورسنت، گرافین اکسید، ترومبین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A label-free THA as sensing platform is designed for thrombin detection.
• THA functions as recognition element and a trigger for fluorescence signal.
• The detection takes place in homogeneous solution.
• The sensing platform shows low detection limit and excellent selectivity.
• The label-free THA can be applied in detection of a wide range of analytes.

The design of a label-free aptamer for separation of recognition sequence from signal reporter is significant to ensure the high-efficiency affinity between aptamer and target. This work develops a label-free triple-helix aptamer (THA) as sensing platform for “signal-on” fluorescent detection of thrombin. THA was composed of aptamer sequence and help DNA 1 (H1), which contained the complementary sequence of hexachloro-fluorescein (HEX) labeled help DNA 2 (H2). The specific recognition event between aptamer and thrombin triggered the dismission of THA to release H1. The released H1 then reacted with the signal probe of H2/graphene oxide (GO) nanocomposite to form H1-H2 duplex, leading to the fluorescence recovery of H2 due to the detachment of H1-H2 duplex from the surface of GO. With employment of THA as a signal transducer and GO as a “superquencher”, this method shows a sensitive response to thrombin with a wide concentration range from 5 to 1200 nM. The limit of detection is 1.8 nM (S/N=3) with excellent selectivity. Considering the universality of THA, the proposed aptasensor would provide a platform for homogeneous fluorescent detection of a wide range of analytes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 132, 15 January 2015, Pages 387–391
نویسندگان
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