کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242031 1495803 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free triple-helix aptamer as sensing platform for “signal-on” fluorescent detection of thrombin
ترجمه فارسی عنوان
اپلامر سه گانه ایلام بدون برچسب بدون نیاز به برچسب به عنوان یک پلت فرم سنجش برای سیگنال؟ تشخیص فلورسنت ترومبین
کلمات کلیدی
سه گانه اپلمر، برچسب رایگان، تشخیص فلورسنت، گرافین اکسید، ترومبین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A label-free THA as sensing platform is designed for thrombin detection.
• THA functions as recognition element and a trigger for fluorescence signal.
• The detection takes place in homogeneous solution.
• The sensing platform shows low detection limit and excellent selectivity.
• The label-free THA can be applied in detection of a wide range of analytes.

The design of a label-free aptamer for separation of recognition sequence from signal reporter is significant to ensure the high-efficiency affinity between aptamer and target. This work develops a label-free triple-helix aptamer (THA) as sensing platform for “signal-on” fluorescent detection of thrombin. THA was composed of aptamer sequence and help DNA 1 (H1), which contained the complementary sequence of hexachloro-fluorescein (HEX) labeled help DNA 2 (H2). The specific recognition event between aptamer and thrombin triggered the dismission of THA to release H1. The released H1 then reacted with the signal probe of H2/graphene oxide (GO) nanocomposite to form H1-H2 duplex, leading to the fluorescence recovery of H2 due to the detachment of H1-H2 duplex from the surface of GO. With employment of THA as a signal transducer and GO as a “superquencher”, this method shows a sensitive response to thrombin with a wide concentration range from 5 to 1200 nM. The limit of detection is 1.8 nM (S/N=3) with excellent selectivity. Considering the universality of THA, the proposed aptasensor would provide a platform for homogeneous fluorescent detection of a wide range of analytes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 132, 15 January 2015, Pages 387–391
نویسندگان
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