کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242189 1495785 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reusable potentiometric screen-printed sensor and label-free aptasensor with pseudo-reference electrode for determination of tryptophan in the presence of tyrosine
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Reusable potentiometric screen-printed sensor and label-free aptasensor with pseudo-reference electrode for determination of tryptophan in the presence of tyrosine
چکیده انگلیسی


• Tryptophan sensor and aptasensor were developed using screen-printed electrodes.
• Ag pseudo-reference electrode was used as an alternative to Ag/AgCl electrode.
• Selectivity and LOD of trytophan biosensor were improved by specific aptamer.
• The proposed sensor/aptasensor can determine tryptophan in biological samples.
• The aptasensor can be integrated with lab-on-a-chip and microfluidic systems.

Analysis of l-tryptophan (Trp) in biological samples has great importance for biomedical studies. Amino acid tyrosine (Tyr) that usually coexist with Trp in biological fluids can significantly interfere with reliable determination of Trp. In the current study, we demonstrate the development of two ultra-sensitive electrochemical sensor and label-free aptasensor for selective analysis of Trp in biological samples (i.e., cow's milk and human plasma, saliva and urine samples). In addition, without using AgCl/KCl, an Ag pseudo-reference screen printed electrode (Ag-PR-SPE) was exploited as a reference electrode. To prepare the engineered Trp sensor/aptasensor, a gold SPE was first modified with multiwall carbon nanotube (MWCNT-AuSPE) and then armed with Trp aptamer molecules (Apt-MWCNT-AuSPE). The prepared sensors were characterized using constant current-potentiometric stripping analysis (CC-PSA) and electrochemical impedance spectroscopy (EIS). The MWCNT-AuSPE and Apt-MWCNT-AuSPE were compared with respect to the linear detection range, limit of detection (LOD), accuracy, precision, repeatability. MWCNT-AuSPE and Apt-MWCNT-AuSPE demonstrate fast near-Nernstian response for PSA of Trp over the concentration ranging from 1.0×10−9 to 2.0×10−4 mol L−1 and 1.0×10−11 to 1.0×10−4 mol L−1 with detection limits of 3.6×10−10 mol L−1 and 4.9×10−12 mol L−1, respectively. Common interfering species present in the biological fluids (i.e., tyrosine, uric acid, ascorbic acid) showed no effects on the determination of Trp using CC-PSA. MWCNT-AuSPE and Apt-MWCNT-AuSPE represented well reproducibility and great precision with relative standard deviation (RSD) of 2.9% and 5.3% respectively. In comparison with the MWCNT-AuSPE, Apt-MWCNT-AuSPE provided higher sensitivity, selectivity and accuracy of Trp detection in real samples. Based on these findings, we propose the developed Apt-MWCNT-AuSPE as a simple detection method for analysis of Trp in biological samples.

Gold screen printed electrode was first modified with (a) multiwall carbon nanotube and then deposited by (b) tryptophan-specific aptamer molecules. These analytical tools based on potentiometric stripping analysis were used for determination of tryptophan in real samples, and the potentiometric responses were compared with that of (c) the conventional three-electrode system.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 150, 1 April 2016, Pages 425–433
نویسندگان
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