A disposable chitosan-modified carbon fiber electrode for dengue virus envelope protein detection

A chitosan-modified carbon fiber electrode (CFE) for dengue virus envelope protein (DENV) was developed. Antibodies against DENV were covalently immobilized on the chitosan (CHIT) matrix after activation with sodium periodate. Cyclic voltammetries and scanning electron microscopies analysis were performed to monitor steps involved in the CFE surface modification. Amperometric response of the competitive immunoassays was generated by hydrogen peroxide reaction with the peroxidase conjugated to DENV and 2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as mediator. The immunosensor showed a lower limit of detection for DENV (0.94 ng mL−1) than previously described and a linear range from 1.0 to 175 ng mL−1, in concentration levels clinically relevant for dengue virus diagnosis. The intra- and inter-assay were respectively 5.8% and 3.6%. The unique and simple design of this immunoassay format provides an economical alternative for the manufacture of other sensitive sensors.

► A disposable electrode based on chitosan modified carbon fiber was developed to detect protein E of dengue virus.
► The use of 2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as mediator for amperometric response showed a good strategy for peroxidase enzyme sensors.
► The detection limit was lower than previously described to protein E.
► The intra- and inter-assay accuracy and precision, determined as relative error and relative standard deviation, respectively were 5.8% and 3.6%.
► This proposed CFE represents a low cost potential tool for envelope protein DENV detection with a good sensitivity.