کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242391 969632 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology
چکیده انگلیسی

A new suspension array technology is proposed for the simultaneous quantitative determination of five antibiotics—tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol in milk. A novel treatment of milk samples for suspension array with diethyl ether was performed which greatly reduced the interference of the disturbing components in milk on the reaction results with no significant effect on detection sensitivity. Compared with using biotin labeled monoclonal antibody, using of secondary antibody–biotin make the detection sensitivity further improved. The minimum detectable concentration in samples of tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol were 0.3, 1.5, 4, 20, and 25 ng/ml, respectively, and the working ranges of samples were 6–400, 7–300, 8–200, 90–3000 and 70–8000 ng/ml, respectively. The mean recovery was 89.38–113.73% with a standard deviation within 16.62%. The suspension assay technology is powerful for the fast quantitative analysis of multi-antibiotics residue in milk.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 2, 15 August 2011, Pages 1160–1165
نویسندگان
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