کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242674 1645368 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antibiotic immunosensing: Determination of sulfathiazole in water and honey
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Antibiotic immunosensing: Determination of sulfathiazole in water and honey
چکیده انگلیسی

The development of two sensitive and selective immunosensors for sulfathiazole, using immunoreagents – haptens, polyclonal antibodies, enzyme conjugates – previously obtained and characterized, is presented. One of them is based on the competitive immunocomplex capture format making use of an immobilized protein A/G sorbent, while the other employs a restricted access support in a novel homogeneous–heterogeneous (HH) assay mode. Maximum sensitivity, achieved with a total assay time of 18 min for the capture sensor, is traduced in a dynamic range from 0.4 to 24 μg L−1, with a lower limit of detection of 0.11 μg L−1, increasing to 1.2 μg L−1 when employing an accelerated capture assay protocol that yields a sampling rate of 7 cycles per hour. The HH sensor shows the fastest response, performing each whole assay in only 2 min, with a limit of detection of 0.85 and a measurement interval of 3.9–181.0 μg L−1, and with no need of support regeneration. Immunosensors are selective for sufathiazole, and only sulfamethoxypyridazine, sulfamethizole and sulfapyridine show non-negligible cross-reactivity, the same as in ELISA batch immunoassay. The application of the developed systems to the analysis of water, with no sample treatment, as well as honey samples after solid-phase extraction, demonstrate the reliability of the immunosensing for the monitoring of this type of pollutants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 81, Issues 4–5, 15 June 2010, Pages 1585–1592
نویسندگان
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