کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242837 969648 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modification of a micellar system for amino acid separation by MEKC—Application for amino acid profiling in formulations for parenteral use
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modification of a micellar system for amino acid separation by MEKC—Application for amino acid profiling in formulations for parenteral use
چکیده انگلیسی

The paper proposes a new method for amino acid determination which can be applied for amino acid profiling in solutions for parenteral nutrition. The MEKC method based on a mixed micellar system was developed for the separation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatized amino acids. Background electrolyte was based on tris–borate buffer with high alkaline pH. Sodium dodecyl sulfate micelles were modified using 1,2-hexanediol as a co-surfactant. The effect of the modifier on amino acid migration was studied with respect to hydrophobicity of the analytes. The modifier appeared to be suitable to improve the separation of AQC-tagged amino acids without an adverse effect on buffer ionic strength or EOF velocity. The method was successfully validated and applied for amino acid profiling in medicinal preparations for parenteral nutrition. The results obtained were compared with a reference chromatographic method (amino acid analyser).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 83, Issue 2, 15 December 2010, Pages 513–520
نویسندگان
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