The study of oxidization fluorescence sensor with molecular imprinting polymer and its application for 6-mercaptopurine (6-MP) determination

This paper developed optical fiber sensor based on molecular imprinted polymer as artificial recognition element for the determination of 6-mercaptopurine (6-MP) in human serum. This approach displayed high sensitivity by oxidizing 6-MP to a strong fluorescent compound with H2O2 in the alkaline media. It offered a relatively nice selectivity for 6-MP detection by molecular imprinted polymer's recognition. The relative standard deviation (R.S.D.) was 5% for a same sensor (n = 5) when 6-MP concentration was 1.0 × 10−7 g mL−1 in serum. The developed method was satisfactorily applied to the determination of 6-MP in human serum without any necessity for sample treatment or time-consuming extraction steps prior to the analysis.