کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1243687 | 969668 | 2009 | 6 صفحه PDF | دانلود رایگان |
A sensitive and efficient method was developed for determination of metoprolol in human plasma by gas chromatography–mass spectrometry (GC–MS). Metoprolol and atenolol (internal standard, IS) were extracted from human plasma with a mixture of ethylacetate and diethylether at basic pH with liquid–liquid extraction. Calibration curves were linear over the concentration range 15–500 ng/ml. Intra- and inter-day precision values for metoprolol in human plasma were less than 6.4, and accuracy (relative error) was better than 8.8%. The analytical recovery of metoprolol from human plasma averaged 91.20%. The limits of detection (LOD) and quantification (LOQ) of metoprolol were 5.0 and 15 ng/ml, respectively. Also the developed and validated GC–MS method was successfully applied to three patients with hypertension who had been given an oral tablet of 100 mg metoprolol.
Journal: Talanta - Volume 80, Issue 1, 15 November 2009, Pages 346–351