Three-way analysis of the UPLC–PDA dataset for the multicomponent quantitation of hydrochlorothiazide and olmesartan medoxomil in tablets by parallel factor analysis and three-way partial least squares

• We described a new application of PARAFAC and 3W-PLS1 to the UPLC data arrays.
• Three-way analysis methods were used to quantify two drugs with internal standard.
• Three-way analysis results were comparable with those obtained by traditional UPLC.

An application of parallel factor analysis (PARAFAC) and three-way partial least squares (3W-PLS1) regression models to ultra-performance liquid chromatography–photodiode array detection (UPLC–PDA) data with co-eluted peaks in the same wavelength and time regions was described for the multicomponent quantitation of hydrochlorothiazide (HCT) and olmesartan medoxomil (OLM) in tablets. Three-way dataset of HCT and OLM in their binary mixtures containing telmisartan (IS) as an internal standard was recorded with a UPLC–PDA instrument. Firstly, the PARAFAC algorithm was applied for the decomposition of three-way UPLC–PDA data into the chromatographic, spectral and concentration profiles to quantify the concerned compounds. Secondly, 3W-PLS1 approach was subjected to the decomposition of a tensor consisting of three-way UPLC–PDA data into a set of triads to build 3W-PLS1 regression for the analysis of the same compounds in samples. For the proposed three-way analysis methods in the regression and prediction steps, the applicability and validity of PARAFAC and 3W-PLS1 models were checked by analyzing the synthetic mixture samples, inter-day and intra-day samples, and standard addition samples containing HCT and OLM. Two different three-way analysis methods, PARAFAC and 3W-PLS1, were successfully applied to the quantitative estimation of the solid dosage form containing HCT and OLM. Regression and prediction results provided from three-way analysis were compared with those obtained by traditional UPLC method.

Three-dimensional UPLC plot of hydrochlorothiazide and olmesartan medoxomil with telmisartan as internal standard and its corresponding chromatographic and spectral profiles obtained by the application of the PARAFAC model.Figure optionsDownload as PowerPoint slide