کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1245079 | 969711 | 2011 | 7 صفحه PDF | دانلود رایگان |
A novel fluorescent probe is designed and synthesized for the determination of cysteine in biological samples by incorporating 2,4-dinitrobenzenesulfonyl (DBS) group as a quencher into the BODIPY skeleton. The BODIPY-based probe itself shows weak fluorescence due to the strong intramolecular charge transfer process. Upon reaction with cysteine, however, the probe produces a rapid and large fluorescence enhancement through the removal of the DBS unit by nucleophilic aromatic substitution. This valuable property leads to the development of a new and simple method for cysteine assay. Under the optimized conditions, the fluorescence enhancement value is directly proportional to the concentration of cysteine in the range 2–12 μM, with a detection limit of 30 nM (S/N = 3). The applicability of the developed method has been successfully demonstrated on the determination of non-protein cysteine in human serum. Compared to most of the existing fluorescent probes proposed for cysteine, the BODIPY-based one exhibits an excellent overall performance in terms of selectivity, sensitivity and simplicity.
Journal: Talanta - Volume 83, Issue 3, 15 January 2011, Pages 1050–1056