کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1245414 969720 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time monitoring of double-stranded DNA cleavage using molecular beacons
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Real-time monitoring of double-stranded DNA cleavage using molecular beacons
چکیده انگلیسی

Traditional methods to assay enzymatic cleavage of DNA are discontinuous, time-consuming and laborious. Here, we report a new approach for real-time monitoring of double-stranded DNA cleavage by restriction endonuclease based on nucleic acid ligation using molecular beacon. Upon cleavage of DNA, the cleavage product can be ligated by DNA ligase, which results in a fluorescence enhancement of the molecular beacon. This method permits real-time monitoring of DNA cleavage and makes it easy to characterize the activity of restriction endonuclease and to study the cleavage reaction kinetics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 76, Issue 2, 15 July 2008, Pages 458–461
نویسندگان
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