کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1245479 969724 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrophoretic separations of neuromediators on microfluidic devices
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrophoretic separations of neuromediators on microfluidic devices
چکیده انگلیسی

In the present work, on-chip capillary electrophoresis for the separation of neuromediators is demonstrated. The influence of separation buffer (composition, pH, SDS additive), on-chip electrokinetic sample stacking, and surface pretreatment of the PDMS–PDMS and hybrid PDMS–glass devices on the electrokinetic characteristics of microfluidics (νeo, μeo, ζ) and separation performance of on-chip capillary electrophoresis of neuromediators have been investigated. It is demonstrated that for the effective separation of neuropeptides on elastomer-based microfluidic devices, on-chip sample stacking is necessary. Field-amplified sample stacking for electroosmotic flow supported on-chip separations of neuromediators and without special design of the sample injection scheme has been demonstrated. Electrophoretic separations of fluorescently labeled analytes have been achieved within tens of seconds at injection volumes of about 110 pL, with plate numbers varying from <1000 to ∼22,000. These results demonstrate that on-chip separation methods with hybrid PDMS–glass devices are perspective for the analysis of (neuro)peptides in small volumes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 70, Issue 3, 15 October 2006, Pages 489–498
نویسندگان
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