کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246009 969742 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An optical immunosensor for rapid vitellogenin detection in plasma from carp (Cyprinus carpio)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An optical immunosensor for rapid vitellogenin detection in plasma from carp (Cyprinus carpio)
چکیده انگلیسی

Vitellogenin (vtg) has proven to be a sensitive and simple biomarker for assessing exposure of fish to environmental estrogens. The aim of this work was to develop a rapid, in the order of minutes, screening method for the detection of fish vtg. The surface plasmon resonance technique (Biacore X™) was coupled with immunodetection for the determination of fish vtg in plasma and mucus from carp (Cyprinus carpio). Monoclonal anti-vtg antibodies were linked on the sensor surface through chemical cross-linking via a capturing antibody. A simple regeneration process allowed the reuse of the sensor surface. Sensor optimisation was carried out using carp vtg. The developed immunosensor was tested with vtg spiked samples and with plasma and mucus from fish exposed to 17β-estradiol (E2). Vitellogenin could be detected in the ppm range in buffer as well as in plasma and mucus. Good discrimination between control and exposed samples was obtained. The results were compared with ELISA and a correlation coefficient of R2 = 0.85 (n = 9) between the two methods indicated that the immunochemical biosensor could be used for the analysis of vtg in fish plasma samples. The assay time was 20 min hence allowing for rapid sample screening.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 72, Issue 2, 30 April 2007, Pages 785–790
نویسندگان
, , , , , , ,