کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1246292 | 969751 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS](/preview/png/1246292.png)
4-Nonylphenol (NP) and octylphenol (OP) were measured by direct ELISA in both laboratory-fortified and surface water samples collected monthly from 10 rivers. In this procedure, samples were concentrated by solid phase extraction (SPE) using Lichrolut RP-18 sorbent with good recoveries obtained for both LC–MS and ELISA, giving a low level of detection (LOD) at the range of low μg L−1 and good reproducibility. Analysis of 40 surface water samples demonstrated that the ELISA may be a useful screening tool for the determination of the alkylphenols in surface water matrices. The concentration of NP and OP in surface waters ranged from 0.11 to 6.58 μg L−1. A good correlation of results obtained by ELISA and LC–MS within the concentration range of 0.08–6.86 μg L−1 was found in the river samples [R2 = 0.924, n = 39]. The influence of various factors on assay determination was also discussed.
Journal: Talanta - Volume 70, Issue 4, 15 November 2006, Pages 745–751