کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246404 969754 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of concanavalin A–mannose interaction on the electrode covered with collagen film
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of concanavalin A–mannose interaction on the electrode covered with collagen film
چکیده انگلیسی

An electrode covered with a lectin/collagen film was constructed to investigate whether the film was usable as a reaction field of binding between the lectin and sugar. The protein–sugar binding on cell surface plays an important role to various physiologic processes. The film is considered to be a cell surface, due to its biocompatibility. The immobilization of concanavalin A (Con A) which is one of proteins was attempted by an electrostatic interaction of the protonated functional groups of film to the negative charged Con A. The merit of this immobilization is that the interaction hardly causes any changes in the protein structure. Because Con A recognizes mannose moiety, the mannose was labeled with an electroactive compound. The binding was estimated from the changes of the electrode response based on the holding of electroactive moiety in the binding site of Con A to the mannose moiety. However, the electrode responses of glucose and galactose labeled with the same substance did not change. The result shows that Con A is immobilized on the film and combines with labeled mannose. Therefore, it is clear that the collagen film is suitable as the reaction field to evaluate the protein–sugar binding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 68, Issue 4, 15 February 2006, Pages 1176–1181
نویسندگان
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